Protein NMR and Mass Spectrometry

A host of additional protein NMR and mass spectrometry facilities are available to CSBWFU investigators in laboratories on both the WFUSM Bowman Gray Campus and the Reynolda Campus. The Department of Biochemistry houses a Bruker Avance 600 MHz NMR spectrometer equipped with 3-channel + 2H amplifiers and triple-axis gradients. Available probes include an inverse-detect, triple-resonance CryoProbe with Z-axis gradients, an inverse-detect triple-resonance TXI probe with 3-axis gradients, an inverse-detect BBI probe with Z-axis gradients, a direct-detect 13C{1H} probe and a 1H{13C} HR-MAS probe. In addition, the Department of Chemistry houses Bruker Avance 500 and 300 MHz NMR spectrometers; the 500 MHz spectrometer is equipped with an inverse-detect triple-resonance TBI probe with Z-gradients and a broadband observe probe. The 300 MHz spectrometer is equipped with an automatic sample changer for high-throughput use.

In addition to the MALDI-TOF and ion-trap mass spectrometers at the BGTC site, the Mass Spectrometry Facility in the Department of Biochemistry (in the Piedmont Triad Research Park) houses a Waters/Micromass Q-TOF APcI US mass spectrometer with an Advion Nanomate Triversa source, a Finnigan Trace GC-MS, and a Quantum TSQ triple quad MS with ESI and nanospray sources.