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Description
DNA sequencing is performed on the Applied Biosystems Model 3100 Genetic Analyzer
and is offered on a fee-for-service basis. Samples submitted for sequencing and
automated analysis undergo a cycle sequencing reaction that uses ABI Big DyeTM
Terminator Chemistry. The fluorescently labeled products are separated from reaction reagents
and analyzed by capillary electrophoresis. The DNA sequence is automatically analyzed
by the 3100 analysis software. Typical read lengths are 600 - 650 bases for high quality
DNA templates and primers.
After sequencing is completed the data is placed in individual folders for each user and posted on a secure web site.